BMP2 rs1005464 is associated with mandibular condyle size variation.

This study aimed to evaluate the association between single nucleotide polymorphisms (SNPs) in endochondral development-related genes and mandibular condyle shape, size, volume, and symmetry traits. Cone-beam Computed Tomographies and genomic DNA from 118 individuals were evaluated (age range: 15-66 years). Data from twelve 3D landmarks on mandibular condyles were submitted to morphometric analyses including Procrustes fit, principal component analysis, and estimation of centroid sizes and fluctuating asymmetry scores. Condylar volumes were additionally measured. Seven SNPs across BMP2, BMP4, RUNX2 and SMAD6 were genotyped. Linear models were fit to evaluate the effect of the SNPs on the mandibular condyles' quantitative traits. Only the association between BMP2 rs1005464 and centroid size remained significant after adjusting to account for the false discovery rate due to multiple testing. Individuals carrying at least one A allele for this SNP showed larger condylar size than common homozygotes GG (ß = 0.043; 95% CI: 0.014-0.071; P value = 0.028). The model including BMP2 rs1005464, age and sex of the participants explained 17% of the variation in condylar size. Shape, volume, and symmetry were not associated with the evaluated SNPs. These results suggest that BMP2 rs1005464 might be associated with variation in the mandibular condyles size.

COVID-19 and Hypertensive Disorders of Pregnancy (HDP): A Portuguese Multicentric Retrospective Cohort Study of HDP in SARS-CoV-2 Infected Patients.

BACKGROUND:  An increased incidence of hypertensive disorders of pregnancy (HDP) has been reported among pregnant women infected by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the pathogen behind coronavirus disease-19 (COVID-19). Although it is primarily a respiratory infection, the extra-pulmonary manifestations of COVID-19 mimic those found in preeclampsia (PE). Moreover, the two conditions share common risk factors and pathological mechanisms, hindering the ability to understand the interaction between them. Current literature on this topic is controversial and as there is an overlap of clinical and laboratory findings, HDP can be an overreported outcome in pregnant women with COVID-19. The aim of our study is to assess whether there is an association between maternal SARS-CoV-2 infection and HDP. METHODS: We designed a multicenter retrospective cohort study with data collected from five maternity hospitals in Almada, Porto, Lisboa, Penafiel and Coimbra, Portugal, between March 2020 and March 2021. We obtained a sample of 789 pregnant women who were followed up or delivered their babies in one of the participating centers. Each pregnant woman who tested positive for SARS-CoV-2 on a real-time polymerase chain reaction test -- exposure group (n= 263), was paired with two negative pregnant women (1:2), who received the same antenatal care and had similar gestational age and parity -- control group (n=526). Data were collected on maternal characteristics, medical history, obstetric outcomes, and delivery.  Outcomes: The primary outcome of our study is to assess the incidence of HDP in pregnant women infected and not infected by SARS-CoV-2. The secondary outcomes of our study are to assess the incidence of HDP across all COVID-19 severity subgroups and to assess whether SARS-CoV-2 infection in pregnancy modified the odds of a set of risk factors developing HDP.  Results: There was a slightly increased, but not statistically significant, incidence of PE (relative risk, RR, 1.33; 95% confidence interval, CI 0.68-2.57) in the SARS-CoV-2 positive group. There was no statistically significant association between having COVID-19 in pregnancy and developing PE/eclampsia/ hemolysis, elevated liver enzymes, and low platelets, HELLP syndrome [X2(1) = 0.732; p = 0.392] as well as developing gestational hypertension (GH) [X2(1) = 0.039; p = 1]. There was no statistically significant association [X2(2) = 0.402; p = 0.875), [X2(2) = 1.529; p = 0.435] between COVID-19 severity and incidence of HDP. The SARS-CoV-2 infection did not modify the odds of each maternal risk factor causing HDP.  Conclusion: Our study did not demonstrate an association between maternal COVID-19 and HDP. We did not observe a significantly increased incidence of HDP in pregnant women infected by SARS-CoV-2. As current literature is controversial on this topic, clinicians should be aware that HDP is a possible complication of maternal SARS-CoV-2 infection and further research studies urge to better assess the association between COVID-19 in pregnancy and HDP.

Patient perception of orthodontists with different stereotypes: development, psychometric properties, and application of an assessment instrument.

INTRODUCTION: This research aimed to develop and validate a questionnaire that considers social judgments and, through its application, assesses whether the presence of piercings, tattoos, beards, and loose hair visible in the face and neck region reflect on the patient perception regarding characteristics of the orthodontist's professional scope. METHODS: The developed questionnaire had its psychometric properties evaluated and was applied to 220 young people and adults, aged 18-70 years, who responded according to their perception of 12 manipulated images of orthodontists with different stereotypes, considering the following items: hygiene, care, punctuality, experience and knowledge in orthodontics. RESULTS: The developed questionnaire showed high acceptability, confirmed reliability and validity of the construct, and satisfactory internal consistency. The stereotype interfered with the perception of professionalism (P <0.01), in which the orthodontist with combined characteristics (beard/loose hair, piercing, earrings, and tattoo) obtained the lowest mean scores, both for each item of the questionnaire individually (P <0.01) and in total (P <0.01). Health professional (P = 0.427) and educational level (P = 0.285) status did not influence the perception; however, there was a significant difference for the age groups (P = 0.041). CONCLUSIONS: The developed questionnaire proved to be valid and reliable for application. Orthodontists with piercings and tattoos on the face and neck were considered less professional. Being a health professional and educational level status did not influence the perception. However, older subjects (aged 51-70 years) were more critical in judging stereotypes than younger subjects (aged 18-30 years).

Emoji as promising tools for emotional evaluation in orthodontics.

BACKGROUND: Emoji are pictograms frequently used in social networks capable of expressing emotions. These tools can provide insights into people's behavior that could not be obtained with the use of textual communication. Recently, emoji have been introduced to various research fields as successful alternatives to word-based questionnaires for measure emotional responses. The objective of this study was to preliminarily evaluate the discriminating ability and relationship of these tools with different occlusal conditions/malocclusions. METHODS: Online surveys were applied to adult individuals (n = 201; mean age = 27.4 ± 5.7; 37.3% males, 62.7% females). Subjects issued acceptance scores (10-point scale) and expressed their emotional status using a 30-emoji list in relation to nine occlusal conditions: C1-crowding, C2-anterior open bite, C3-interincisal diastema, C4-increased overjet + deep bite (Class II div. 1), C5-anterior crossbite (Class III), C6-ideal occlusion, C7-unilateral posterior crossbite, C8-anterior open bite plus bilateral posterior crossbite plus crowding, and C9-deep bite (Class II div. 2). Cochran's Q and McNemar tests were used to compare the frequencies of choice of emoji between conditions. Correspondence analyses were applied to assess the association between occlusal conditions and emoji. Kendall's correlation coefficient was calculated to evaluate the relationship between mean acceptance scores and frequency counts of each emoji. RESULTS: The frequency of choice between conditions showed a significant difference for 25 of the 30 emoji (P < 0.05), indicating an adequate discriminating ability of these tools. Emoji were grouped predominantly based on their emotional valence (positive/negative) and arousal/activation (high/low). Positive emoji were associated with the most accepted conditions (i.e., C6, C3), while negative emoji with the most rejected ones (i.e., C8, C1, C2). Although only weak, positive correlations between acceptance and positively valenced emoji, and negative correlations between acceptance and negatively valenced emoji were observed (P < 0.05). CONCLUSIONS: Emoji have an adequate discriminatory ability and would allow determining emotional profiles in the face of different occlusal conditions. Further research is necessary to consolidate the use of these tools in an instrument that allows measuring emotional responses.

Collagen membrane and distraction osteogenesis for correcting alveolar bone defects: An animal pilot study.

BACKGROUND: The objective of this study was to evaluate the applicability of distraction osteogenesis with or without collagen membrane coverage to approximate the bone surfaces surrounding alveolar defects in pigs. METHODS: The sample comprised six pigs. One animal was control, and in the other five, alveolar bone defects were created and distraction osteogenesis was carried out to approximate adjacent bone surfaces in the defect site. Resorbable membranes were placed to cover the alveolar defects on one palatal side of the experimental animals. Radiographs were taken, and the histology was studied in each bone defect site at the 2nd, 9th, 16th, 23rd, and 46th days of healing. RESULTS: The radiographs showed that the alveolar bone defects were successfully corrected. Initial osteogenesis was delayed, but bone bridge formation was complete in the membrane-covered samples at the 46th day of healing. In the other group, bone bridge formation was incomplete at the end of the study. CONCLUSIONS: Distraction osteogenesis was effective in approximating transported bones to host bones. The resorbable membranes produced complete bone bridges in the approximation sites.

Prenatal Exposure to Metals and Neurodevelopment in Infants at Six Months: Rio Birth Cohort Study of Environmental Exposure and Childhood Development (PIPA Project).

The PIPA Project is a prospective birth cohort study based in Rio de Janeiro, Brazil, whose pilot study was carried out between October 2017 and August 2018. Arsenic (As), cadmium (Cd), lead (Pb), and mercury (Hg) concentrations were determined in maternal (n = 49) and umbilical cord blood (n = 46). The Denver Developmental Screening Test II (DDST-II) was applied in 50 six-month-old infants. Metals were detected in 100% of the mother and newborn samples above the limits of detection. Maternal blood lead concentrations were higher in premature newborns (GM: 5.72 µg/dL; p = 0.05). One-third of the infants (n = 17-35.4%) exhibited at least one fail in the neurodevelopment evaluation (fail group). Maternal blood arsenic concentrations were significantly (p = 0.03) higher in the "fail group" (GM: 11.85 µg/L) compared to infants who did not fail (not fail group) (GM: 8.47 µg/L). Maternal and umbilical cord blood arsenic concentrations were higher in all Denver Test's domains in the "fail group", albeit non-statistically significant, showing a tendency for the gross motor domain and maternal blood (p = 0.07). These findings indicate the need to further investigate the toxic effects of prenatal exposure to metals on infant neurodevelopment.

Communication strategies used by adolescents with autism spectrum disorder and health professionals during treatment.

Background: Autism spectrum disorder (ASD) is a developmental disorder, which affects social communicative capabilities. The research study has shown that ASD studies are focused on young children, excluding adolescents and adults: and it is understudied in the context of South Africa. Objective: This study examined the interactional communication strategies of adolescents with ASD and health professionals during different treatment consultations to identify the interactional styles and communication strategies utilised by adolescents with ASD and their respective healthcare professionals in a variety of scenarios in order to generate management strategies for future healthcare professional communication training. Method: A multi-case study design with a qualitative research approach has been used. Four adolescents with a moderate form of ASD and four health practitioners were interviewed. Participants were chosen by purpose and snowball sampling. Semi-structured, open-ended interviews were used for health professionals to collect information on the various interaction types and communication methods used, as well as their interpretations of these methods. Conversely, adapted face-to-face interviews were used to collect similar knowledge from adolescents themselves. The findings were qualitatively analysed on a case-by-case and cross-case basis by thematic analysis techniques. Results: The findings indicated that ASD adolescents have interaction types that influence intervention to various degrees. In comparison to motor therapies such as occupational therapy and physiotherapy, interaction types have a greater impact on psychiatry and psychology, which depend mainly on verbal communication. Intuitively, to promote contact with these teens, all health practitioners changed their own interaction styles. They used techniques of clarification and repair. The therapists shared the intention to learn a range of successful ways to strengthen future experiences with ASD between themselves and adolescents. Conclusion: The findings indicate that practitioners can benefit from altering their interaction styles, and that approaches for promoting successful interactions and in establishing rapport could be shared with other professionals in the future.

Semisolid formulations based on solid-in-oil-in-water systems containing proteins

Abstract In recent years, improvements have been made, through biotechnological processes, in the production and development of peptides capable of increasing collagen and elastin synthesis for anti-aging skin care. However, proteins have many limitations due to their structural, chemical and physical fragility to external aggressions, which may cause conformational changes, leading to loss of biological activity. Therefore, it is important to create delivery systems that protect these biomolecules from damage, allowing them to reach their target. This work aimed to develop a system able to carry bovine serum albumin (BSA), used as a model of a protein, and to incorporate this system in a semisolid formulation suitable for skin application. A microemulgel based on a solid-in-oil-in-water (S/O/W) microemulsion was prepared. Firstly, the association efficiency (AE) of lyophilized BSA-sucrose ester complex and the size of S/O nanodispersion were assessed; then, the characterization and stability evaluation of the final semisolid formulation through evaluation of pH, texture and rheological behavior were performed. The average value of AE was 54.74% ± 2.17. It was possible to develop an S/O/W microemulsion, which allowed the subsequent development of an S/O/W microemulgel that assured suitable pH, texture and rheological characteristics for skin application.

Orthodontic camouflage as a treatment alternative for skeletal Class III.

INTRODUCTION: Skeletal Class III malocclusion is a deformity of complex treatment, with few intervention alternatives, which are further limited in nongrowing patients. In most cases, orthognathic surgery is the ideal treatment for adults, an option often refused by patients. Mild to moderate skeletal Class III malocclusions and acceptable facial esthetics can benefit from a course of treatment in which dental movements are used to compensate for the skeletal discrepancy. OBJECTIVE: This study aimed to discuss orthodontic camouflage as an option for adult patients with Class III malocclusion, emphasizing its indications, implications and expected results.

HbA1c as a predictor of postpartum diabetes mellitus after gestational diabetes mellitus.

AIMS: Women diagnosed with Gestational Diabetes Mellitus (GDM) should be evaluated postpartum with an Oral Glucose Tolerance Test (OGTT). Nevertheless, women frequently fail to it. We intend to evaluate the performance of third trimester HbA1c in the prediction of postpartum diabetes mellitus (PDM). METHODS: We conducted a retrospective study of 10245 women with GDM based on the National Registry of GDM. A receiver-operating characteristic (ROC) curve was plotted to evaluate the diagnostic performance of third trimester HbA1c in PDM prediction. RESULTS: The mean third trimester HbA1c level was 5.81% (SD 0.69%) in women who developed PDM, 5.40% (SD 0.52%) in women with pre-diabetes and 5.21% (SD 0.43%) in women with normal glucose tolerance in postpartum OGTT, with statistically significant differences (p < 0.0001). As to the ROC curve ability to predict PDM was fair, with an optimal cut-off point of HbA1c of 5.4%. Women presenting HbA1c values ≥ 5.4% were 6.1 times more likely to develop PDM. CONCLUSIONS: A third trimester HbA1c level ≥5.4% is associated with a significant higher risk of PDM (p < 0.0001). It could be used as a reliable tool for screening women with GDM and detect who will benefit the most from a close follow-up after pregnancy.

Stress and displacement of mini-implants and appliance in Mini-implant Assisted Rapid Palatal Expansion: analysis by finite element method.

OBJECTIVE: In this study, simulations were performed by the finite element method (FEM) to determine the tension and displacement in mini-implants and in expander appliance during rapid maxillary expansion, by varying the number and location of the mini-implants. METHODS: For the computational simulation, a three-dimensional mesh was used for the maxilla, mini-implants and expander appliance. Comparisons were made on six different Mini-implant Assisted Rapid Palatal Expander (MARPE) configurations, by varying the amount and location of mini-implants. A closed suture was design and received two activations of 0.25 mm, and an open suture had a 0.5-mm aperture that received 20 activations, also of 0.25 mm. RESULTS: For the closed suture, the maximum displacement values in the mini-implants were between 0.253 and 0.280 mm, and stress was between 1,348.9 and 2,948.2 MPa; in the expander appliance, the displacement values were between 0.256 and 0.281 mm, and stress was between 738.52 and 1,207.6 MPa. For the open suture, the maximum displacement values in the mini-implants were between 2.57 and 2.79 mm, and stress was between 5,765.3 and 10,366 MPa; in the appliance, the maximum displacements was between 2.53 and 2.89 mm, and stress was between 4,859.7 and 9,157.4 MPa. CONCLUSIONS: There were higher stress concentrations in the mini-implant than in the expander arm. In the simulations with a configuration of three mini-implants, stress overload was observed in the isolated mini-implant. Displacements of the mini-implants and arms of the appliance were similar in all simulations.

Chromatic analysis of orthodontic resin bonding agents exposed to different antiseptic mouthrinses.

OBJECTIVE: To assess the color of different orthodontic resin bonding agents exposed to three antiseptic mouthrinses for a prolonged time interval (10-year aging simulation). METHODS: 160 specimens were distributed into four groups, according to the orthodontic resin bond agent (Concise, Transbond XT, Transbond Plus Color Change, and Natural Ortho). Each group was exposed to different antiseptic mouthrinses: alcohol-based (Listerine®), alcohol-free (Oral-B®), chlorhexidine (Periogard®) and distilled water as the control. Specimens were submitted to two cycles of staining and artificial aging. Color was evaluated by means of a digital spectrophotometer at the beginning of the experiment and after every cycle. The system used to assess color changes was the CIE L*a*b*. Data was analyzed using the ANOVA and Tukey post-hoc test. RESULTS: After simulation of 10 years of aging, Transbond XT and Natural Ortho composites presented no statistically significant differences in ∆E when exposed to different mouthrinses. The Concise composite specimens exposed to alcohol-free mouthrinse presented a significant difference when compared with specimens from the same group exposed to other antiseptic mouthrinses. Transbond Plus Color Change specimens exposed to chlorhexidine mouthrinse and to alcohol-containing mouthrinse presented a significant difference when compared with the specimens from the group exposed to water and alcohol-free antiseptic. CONCLUSION: All orthodontic resin bonding agents tested presented clinically perceptible color changes when exposed to at least one of the mouthrinses, except for the Natural Ortho composite. The Concise composite exposed to the alcohol-free solution was the resin that presented the highest color change values.

Reasons influencing the preferences of prospective patients and orthodontists for different orthodontic appliances.

OBJECTIVE: To evaluate the reasons influencing the preferences for a certain type of orthodontic appliance over another among prospective patients (PP) and orthodontists. METHODS: A total of 49 PP and 51 orthodontists were asked about their preferences for the following appliances: clear aligners (CA), lingual metallic brackets (LMB), polycrystalline and monocrystalline ceramic brackets, and buccal metallic brackets (BMB). The participants rated the importance of 17 potential reasons that would explain their choices. The reasons that contributed most to these preferences were identified. Non-parametric tests (Fisher's exact, χ2 and Mann-Whitney tests) and multivariate analyses (regression and discriminant analysis) were used to assess the data (α = 0.05). RESULTS: CA and BMB were the most chosen appliances by PP and orthodontists, respectively. LMB was the most rejected option among both groups of participants (p < 0.001). Rates of the importance of pain/discomfort, smile esthetics, finishing details, and feeding/speech impairment showed the highest differences between PP and orthodontists (p < 0.0005). Discriminant analyses showed that individuals who considered treatment time and smile esthetics as more important were more likely to prefer CA, while those who prioritized finishing details and cost were more likely to choose BMB (p < 0.05). CONCLUSIONS: Reasons related to comfort and quality of life during use were considered as more important by PP, while those related to the results and clinical performance of the appliances were considered as more relevant by orthodontists.

Phytochemical profile and ethnopharmacological applications of Lecythidaceae: An overview.

ETHNOPHARMACOLOGICAL RELEVANCE: The family Lecythidaceae has about 25 genera and 400 identified species, which are distributed especially in the pantropical region, mostly found in the tropics of Central and South America, Southeast Asia and Africa. The third most abundant family in Amazonian forests and the genus Eschweilera, with the large number of species in Lecythidaceae. AIM OF THE REVIEW: The present review compiles information since the 1934s about of ethnopharmacology, and chemical constituents of species of Lecythidaceae, as well as a summary of the bioactivities shown by the extracts, fractions, and secondary metabolites. MATERIALS AND METHODS: All relevant information on ethnopharmacology, and chemical constituents of species of Lecythidaceae were gathered from electronic databases including Web of Science, Science Direct, Elsevier, ResearchGate, and Google Scholar. Information was also obtained from local books, PhD. and MSc. Dissertations. RESULTS: The phytochemical diversity of the family was demonstrated with 180 different metabolites that have been reported from 25 species, most of them being triterpenes or flavonoids. The pharmacological studies carried out with the extracts, fractions and compounds showed promising antibacterial, antifungal, anti-inflammatory, antinociceptive, cytotoxic and antioxidant activities. CONCLUSION: The present review provides an insight into ethnopharmacology, phytochemistry and pharmacology of species of Lecythidaceae. Based on the pharmacological studies it has been found that different plant species of Lecythidaceae possess a wide range of bioactivities such as anti-arthritic, anti-inflammatory, antileishmanial, antibacterial and antifungal. These activities are due to the presence of bioactive compounds including triterpenoids and their glycosides derivatives, flavonoids, steroids, sesquiterpenoids, alkaloids, and other compounds. However, there are many plants, which have not been assessed pharmacologically and hence warrant further studies.

FGF10 and FGF13 genetic variation and tooth-size discrepancies.

OBJECTIVES: To explore whether variations in odontogenesis-related genes are associated with tooth-size discrepancies. MATERIALS AND METHODS: Measurements of the width of permanent teeth were obtained from dental casts of 62 orthodontic patients (age 15.65 ± 6.82 years; 29 males and 33 females). Participants were classified according to the anterior and overall Bolton ratios as without tooth-size discrepancy or with maxillary or mandibular tooth-size excess. Genomic DNA extracted from buccal cells was used, and 13 single nucleotide polymorphisms (SNPs) across nine genes were genotyped by polymerase chain reaction using TaqMan chemistry. χ2 or Fisher exact tests were applied to determine the overrepresentation of genotypes/alleles depending on the type of tooth-size discrepancy (α = .05; corrected P value: P < 5.556 × 10-3). Odds ratios (ORs) and their correspondent 95% confidence intervals (CIs) were also calculated to investigate the risk of this phenotype for the SNPs having significant association. RESULTS: Individuals carrying the FGF10 rs900379 T allele were more likely to have larger mandibular teeth (OR = 3.74; 95% CI: 1.65-8.47; P = .002). This effect appeared to be stronger when two copies of the risk allele (TT) were found (recessive model, OR = 6.16; 95% CI: 1.71-22.16; P = .006). On the other hand, FGF13 rs5931572 rare homozygotes (AA, or male A hemizygotes) had increased risk of displaying tooth-size discrepancies when compared with the common homozygotes (GG, or male G hemizygotes; OR = 10.32; 95% CI: 2.20-48.26; P = .003). CONCLUSIONS: The results suggest that FGF10 and FGF13 may contribute to the presence of tooth-size discrepancies.

Effectiveness of methods for cleaning arch wire: an in vitro study

The aim of this study was to evaluate various methods of removing bacterial and fungus biofilm, to simulate orthodontic arch wires cleaning before reinsertion in the patients appliance. Rectangular Nickel Titanium (NiTi), Stainless Steel (SS) and Titanium Molybdenum (TMA) wires were divided into five groups, then contaminated with strains of Streptococcus mutans and Candida albicas. Four segments of each group served as control and were not contaminated. Six cleanings methods were used to remove the biofilm: cotton roll and a chemical agent (chlorhexidine, sodium hypochlorite, 70% alcohol), cotton roll and water, steel woll and immersion on enzymatic detergent. There was a control group not decontaminated Then wires were placed in broth separately, and after an incubation period the optical density (OD) was measured, observing whether there was microbial growth. A wire segment of each subgroup of SS 3M® was taken to the Scanning Electron Microscope (SEM) for visualization of the treatment response. The results were submitted to one-way ANOVA test and Tukey post-test. With the exception of 70% alcohol, the disinfection means behaved similarly regardless the type of wire. Two percent Chlorhexidine and 1% Sodium Hypochlorite totally removed the microorganisms while other agents left a high microbial concentration. Chemical cleaning is necessary to remove biofilm in orthodontic wires; 1% Sodium Hypochlorite and 2% Chlorhexidine are good disinfectants for this purpose.

Deformation of the circummaxillary sutures during acute micro-implant assisted rapid palatal expansion and tooth-supported expansion: An ex vivo study.

INTRODUCTION: This study aimed to assess and compare the deformation that develops in the circummaxillary sutures during activation of micro-implant assisted rapid palatal expander (MARPE) and tooth-supported expander (Hyrax) s, in the rapid maxillary expansion. SETTINGS AND SAMPLE: 7 pigs Sus Scrofa received custom-made MARPE (n = 3) and Hyrax (n = 4) appliances. MATERIAL AND METHODS: The devices were activated 25 times with strain readings captured by strain gauges attached to the following regions: posterior midpalatal suture (MPS), maxilla-premaxilla suture (MPM), maxilla-zygomatic suture (MZ) and maxilla pterygoid-process suture (MPP). The intermolar distance and suture width were measured immediately before activation and at the 20th and 25th activation. ANOVA and Kruskal-Wallis test was applied. RESULTS: The MARPE group presented greater MPS displacement in all measured regions, and one of the devices produced a significant opening (1.7 mm) in the posterior region. The accumulated tension in the MPS was higher compared to the other sutures (P < .05). A MARPE animal presented higher median tension in the MPS region (294.77µÎµ) compared to all other animals except one Hyrax animal (P < .05). Regarding the median tensions of the different activation intervals, the median tension measured during the 16th to 25th activation interval in the Hyrax group was lower than that measured during the first 8 activations, in both the MPS and MZ (P < .05). CONCLUSIONS: MARPE expanders developed more constant tensions during all activations (MPS and MZ), while Hyrax showed lower tension in the 16th to 25th activation.

Chromatic analysis of orthodontic resin bonding agents exposed to different antiseptic mouthrinses

ABSTRACT Objective: To assess the color of different orthodontic resin bonding agents exposed to three antiseptic mouthrinses for a prolonged time interval (10-year aging simulation). Methods: 160 specimens were distributed into four groups, according to the orthodontic resin bond agent (Concise, Transbond XT, Transbond Plus Color Change, and Natural Ortho). Each group was exposed to different antiseptic mouthrinses: alcohol-based (Listerine®), alcohol-free (Oral-B®), chlorhexidine (Periogard®) and distilled water as the control. Specimens were submitted to two cycles of staining and artificial aging. Color was evaluated by means of a digital spectrophotometer at the beginning of the experiment and after every cycle. The system used to assess color changes was the CIE L*a*b*. Data was analyzed using the ANOVA and Tukey post-hoc test. Results: After simulation of 10 years of aging, Transbond XT and Natural Ortho composites presented no statistically significant differences in ∆E when exposed to different mouthrinses. The Concise composite specimens exposed to alcohol-free mouthrinse presented a significant difference when compared with specimens from the same group exposed to other antiseptic mouthrinses. Transbond Plus Color Change specimens exposed to chlorhexidine mouthrinse and to alcohol-containing mouthrinse presented a significant difference when compared with the specimens from the group exposed to water and alcohol-free antiseptic. Conclusion: All orthodontic resin bonding agents tested presented clinically perceptible color changes when exposed to at least one of the mouthrinses, except for the Natural Ortho composite. The Concise composite exposed to the alcohol-free solution was the resin that presented the highest color change values.

RESUMO Objetivo: Avaliar a cor de diferentes resinas ortodônticas expostas a três enxaguantes bucais, por um intervalo de tempo prolongado (simulação de 10 anos de envelhecimento). Métodos: 160 espécimes foram distribuídos em quatro grupos, de acordo com a resina de colagem ortodôntica (Concise, Transbond XT, Transbond Plus Color Change e Natural Ortho). Cada grupo foi exposto a diferentes tipos de enxaguantes bucais: à base de álcool (Listerine®), isento de álcool (Oral-B®), clorexidina (Periogard®) ou água destilada, como controle. Os espécimes foram submetidos a dois ciclos de manchamento e envelhecimento artificial. A cor foi avaliada por meio de um espectrofotômetro digital no início do experimento e após cada ciclo. O sistema utilizado para avaliar as alterações de cor foi o CIE L*a*b*. Os dados foram analisados utilizando-se o teste ANOVA e o teste post-hoc de Tukey. Resultados: Após a simulação de 10 anos de envelhecimento, as resinas Transbond XT e Natural Ortho não apresentaram diferenças estatisticamente significativas no ∆E, quando expostas a diferentes enxaguantes bucais. Os espécimes do grupo Concise expostos ao enxaguante bucal sem álcool apresentaram diferença significativa quando comparados aos espécimes do mesmo grupo expostos aos outros enxaguantes bucais. Os espécimes do grupo Transbond Plus Color Change expostos à clorexidina e ao enxaguante bucal à base de álcool apresentaram diferença significativa quando comparados aos espécimes dos grupos expostos à água e ao enxaguante bucal sem álcool. Conclusão: Todas as resinas ortodônticas avaliadas apresentaram alterações de cor clinicamente perceptíveis quando expostas a pelo menos um dos enxaguantes bucais, com exceção da resina ortodôntica Natural Ortho. A resina Concise exposta à solução isenta de álcool foi a que apresentou os maiores valores de alteração de cor.

Orthodontic camouflage as a treatment alternative for skeletal Class III

ABSTRACT Introduction: Skeletal Class III malocclusion is a deformity of complex treatment, with few intervention alternatives, which are further limited in nongrowing patients. In most cases, orthognathic surgery is the ideal treatment for adults, an option often refused by patients. Mild to moderate skeletal Class III malocclusions and acceptable facial esthetics can benefit from a course of treatment in which dental movements are used to compensate for the skeletal discrepancy. Objective: This study aimed to discuss orthodontic camouflage as an option for adult patients with Class III malocclusion, emphasizing its indications, implications and expected results.

RESUMO Introdução: A má oclusão esquelética de Classe III é uma deformidade de difícil tratamento e com poucas alternativas de intervenção, que ficam ainda mais limitadas em pacientes sem crescimento. Na maior parte dos casos, o tratamento ideal para adultos é a cirurgia ortognática, opção muitas vezes recusada pelo paciente. As más oclusões esqueléticas de Classe III leve a moderada e com estética facial aceitável podem se beneficiar de um plano de tratamento no qual movimentações dentárias são realizadas para compensar a discrepância esquelética. Objetivo: O objetivo do presente estudo foi discutir a camuflagem ortodôntica como opção para pacientes adultos com má oclusão de Classe III, ressaltando suas indicações, implicações e resultados esperados.

Metal mixtures in pregnant women and umbilical cord blood at urban populations-Rio de Janeiro, Brazil.

This study aims to assess interrelationships between serum lead (Pb), cadmium (Cd), arsenic (As), and mercury (Hg) concentrations in pregnant women in their third trimester and umbilical cord blood, while inter-metal correlations were also determined. This study is part of the PIPA project (Childhood and Environmental Pollutant Project), whose pilot study was carried out from October 2017 to August 2018 and will be presented here. Blood samples were obtained from 117 mother-umbilical cord pairs and analyzed concerning metal concentrations. A positive correlation was found between metal concentrations in mother and cord blood (R > 0.7, p < 0.001). The results indicate that mother metal concentrations are able to determine child metal concentrations (p < 0.001). The correlations between maternal blood metal concentrations were positive for all assessed metals except for As and Hg. The strongest correlations in this matrix were observed between Cd and Pb (R = 0.471 p = 0.000), Cd and Hg (R = 0.425 p = 0.000), and Pb and Hg (R = 0.427 p = 0.000). Umbilical cord correlations were lower compared to mother blood correlations. In general, the four analyzed metals displayed significant correlations to serum concentrations in both maternal and cord blood.